Publications

  1. Pool EH*, Glebov-McCloud A*, Lee HN, Hardy JC, Pane V, Herberg FW, Taylor SS, Mehta S, Strack S†, and Zhang J†. Aberrant phase separation of two RIβ neurological disorder mutants leads to mechanistically distinct signaling deficits. *Equal contribution. †Co-corresponding authors. Cell Rep. 2025; 44:115797. PMID 40512625
  2. Lee J*, Lai S*, Yang S, Zhao S, Blanco SA, Lyons AC, Merino-Urteaga R, Ahrens JF, Nguyen NA, Liu H, Liu Z, Lambert GG, Shaner NC, Chen L, Tolias KF, Zhang J, Ha T, and St-Pierre F. Bright and photostable yellow fluorescent proteins for extended imaging. *Equal contribution. Nat Commun. 2025; 16:3241. PMID 40185748
  3. Frei MS†, Sanchez SA*, He X*, Liu L, Schneider F, Wang Z, Hakozaki H, Li Y, Lyons AC, Rohm TV, Olefsky JM, Shi L, Schöneberg J, Fraser SE, Mehta S, Wang Y, and Zhang J†. Far-red chemigenetic kinase biosensors enable multiplexed and super-resolved imaging of signaling networks. *Equal contribution. †Co-corresponding authors. Nat Biotechnol. 2025. PMID 40258957
  4. Sahan AZ*, Chen M*, Su Q, Li Q, Wang D, and Zhang J. Lysosomal PIP 3 revealed by genetically encoded lipid biosensors. *Equal contributions. Proc Natl Acad Sci USA. 2025; 122 (13) e2426929122. PMID 40127277
  5. Gest AMM*, Sahan AZ*, Zhong Y*, Lin W, Mehta S, and Zhang J. Molecular spies in action: Genetically encoded fluorescent biosensors light up cellular signals. *Equal contributions. Chem Rev. 2024; 124(22): 12573–12660. PMID 39535501
  6. Su Q, Zhang J, Lin W, Zhang JF, Newton AC, Mehta S, Yang J, and Zhang J. Sensitive fluorescent biosensor reveals differential subcellular regulation of PKC. Nat Chem Biol. 2024. PMID 39394268
  7. Lin W†, Phatarphekar A, Zhong Y, Liu L, Kwon HB, Gerwick WH, Wang Y, Mehta S, and Zhang J†. Light-gated integrator for highlighting kinase activity in living cells. †Co-corresponding authors. Nat Commun. 2024; 15: 7804. PMID 39242543
  8. Weeks R, Mehta S, and Zhang J. Genetically encodable biosensors for Ras activity. RSC Chem Biol. 2024; 5: 312-320. PMID 38576721
  9. Frei MS, Mehta S, and Zhang J. Next-generation genetically encoded fluorescent biosensors illuminate cell signaling and metabolism. Annu Rev Biophys. 2024; 53. PMID 38346245
  10. Hardy JC, Pool EH, Bruystens JGH, Zhou X, Li Q, Zhou DR, Palay M, Tan G, Chen L, Choi JLC, Lee HN, Strack S, Wang D, Taylor SS, Mehta S, and Zhang J. Molecular determinants and signaling effects of PKA RIα phase separation. Mol Cell. 2024; 84(8): 1570-1584.e7. PMID 38537638
  11. Lyons AC, Mehta S, and Zhang J. Fluorescent biosensors illuminate the spatial regulation of cell signaling across scales. Biochem J. 2023; 480(20): 1693–1717. PMID 37903110
  12. Elias R, Zhu Y, Su Q, Ghirlando R, Zhang J, and Deshmukh L. Reversible phase separation of ESCRT protein ALIX through tyrosine phosphorylation. Sci Adv. 2023; 9(28): eadg3913. PMID 37450591
  13. Posner C, Mehta S, and Zhang J. Fluorescent biosensor imaging meets deterministic mathematical modelling: quantitative investigation of signalling compartmentalization. J Physiol. 2023; 601(19): 4227-4241. PMID 37747358
  14. Greenwald E*, Posner C*, Bharath A, Lyons A, Salmeron C, Sriram K, Wiley SZ, Insel PA†, and Zhang J†. GPCR signaling measurement and drug profiling with an automated live-cell microscopy system. *Equal contribution. †Co-corresponding authors. ACS Sensors. 2023; 8(1): 19-27. PMID 36602887
  15. Schmitt DL, Mehta S, Zhang J. Study of spatiotemporal regulation of kinase signaling using genetically encodable molecular tools. Curr Opin Chem Biol. 2022; 71:102224. PMID 36347198
  16. Kwon Y, Mehta S, Clark M, Walter G, Zhong Y, Lee HN, Sunahara RK, Zhang J. Non-canonical beta-adrenergic activation of ERK at endosomes. Nature. 2022; 611(7934): 173-179. PMID 36289326
  17. Kwon Y, Kim DH, Jeong MG, Hong MT, Park S, Chang Y, Zhou K, Park SY, Zhang J, Ryu SH. Dimerization of beta2-adrenergic receptor is responsible for the constitutive activity subjected to inverse agonism. Cell Chem Biol. 2022; 29(10): 1532-1540. PMID 36167077
  18. Hashizume R, Fujii H, Mehta S, Ota K, Qian Y, Zhu W, Drobizhev M, Nasu Y, Zhang J, Bito H, Campbell RE. A genetically encoded far-red fluorescent calcium ion biosensor derived from a biliverdin-binding protein. Protein Sci. 2022; 31(10): e4440. PMID 36173169
  19. Weeks R, Zhou X, Yuan TL, Zhang J. Fluorescent biosensor for measuring Ras activity in living cells. J Am Chem Soc. 2022; 144(38): 17432-17440. PMID 36122391
  20. Schmitt DL, Curtis SD, Lyons AC, Zhang JF, Chen M, He CY, Mehta S, Shaw RJ, Zhang J. Spatial regulation of AMPK signaling revealed by a sensitive kinase activity reporter. Nat Commun. 2022; 13: 3856. PMID 35790710
  21. Zhong Y, Zhou X, Guan KL, Zhang J. Rheb regulates nuclear mTORC1 activity independent of farnesylation. Cell Chem Biol. 2022; 29(6): 1037-1045.e4. PMID 35294906
  22. Zhou X, Mehta S, Zhang J. AktAR and Akt-STOPS: Genetically encodable molecular tools to visualize and perturb Akt kinase activity at different subcellular locations in living cells. Current Protocols.. 2022; 2: e416. PMID 35532280
  23. Mehta S and Zhang J. Liquid-liquid phase separation drives cellular function and dysfunction in cancer. Nat Rev Cancer. 2022; 22(4): 239-252. PMID 35149762
  24. Hardy JC, Mehta S, Zhang J. Measuring spatiotemporal cAMP dynamics within an endogenous signaling compartment using FluoSTEP-ICUE. Methods Mol Biol. 2022; 2483: 351-366. PMID 35286687.
  25. Zhang JF, Mehta S, Zhang J. Protocol for reading and imaging live-cell PKA activity using ExRai-AKAR2. STAR Protoc. 2022; 3(1): 101071. PMID 35036953
  26. Chen M, Sun T, Zhou X, Zhang J. A sensitive fluorescent Akt biosensor reveals lysosome-selective regulation of lipid second messengers and kinase activity. ACS Cent Sci. 2021; 7(12): 2009-2020. PMID 34963894
  27. Valentine JM, Ahmadian M, Keinan O, Abu-Odeh M, Zhao P, Zhou X, Keller MP, Gao H, Yu RT, Liddle C, Downes M, Zhang J, Lusis AJ, Attie AD, Evans RM, Rydén M, Saltiel AR. β3-adrenergic receptor downregulation leads to adipocyte catecholamine resistance in obesity. J Clin Invest. 2022; 132(2): e153357. PMID 34847077
  28. Lee YU, Posner C, Nie Z, Zhao J, Li S, Bopp SE, Wisna GBM, Ha J, Song C, Zhang J, Yang S, Zhang X, Liu Z. Organic hyperbolic material assisted illumination nanoscopy. Adv Sci. 2021; 8(22): 2102330. PMC8596098
  29. Zhang JZ, Mehta S, Zhang J. Liquid-liquid phase separation: a principal organizer of the cell’s biochemical activity architecture. Trends Pharmacol Sci. 2021; 42(10): 845-856. PMID 34373114
  30. Su Q, Mehta S, Zhang J. Liquid-liquid phase separation: orchestrating cell signaling through time and space. Mol Cell. 2021; 81(20): 4137-4146. PMID 34619090
  31. Lin W, Mo GCH, Mehta S, Zhang J. DrFLINC contextualizes super-resolution activity imaging. J Am Chem Soc. 2021; 143(37): 14951-14955. PMID 34516108
  32. Zhang JZ, Tenner B, Mehta S, Zhang J. Protocol for using Fluorescent Sensors Targeted to Endogenous Proteins (FluoSTEPs) to measure microdomain-specific signaling events. STAR Protoc. 2021; 2(3): 100693. PMID 34467224
  33. Liu L, Limsakul P, Meng X, Huang Y, Harrison RES, Huang TS, Shi Y, Yu Y, Charupanit K, Zhong S, Lu S, Zhang J, Chien S, Sun J, Wang Y. Integration of FRET and sequencing to engineer kinase biosensors from mammalian cell libraries. Nat Commun. 2021; 12(1): 5031. PMID 34413312
  34. Keyes J, Mehta S, Zhang J. Strategies for multiplexed biosensor imaging to study intracellular signaling networks. Methods Mol Biol. 2021; 2350: 1-20. PMID 34331275
  35. Abu-Odeh M, Zhang Y, Reilly SM, Ebadat N, Keinan O, Valentine JM, Hafezi-Bakhtiari M, Ashayer H, Mamoun L, Zhou X, Zhang J, Yu RT, Dai Y, Liddle C, Downes M, Evans RM, Kliewer SA, Mangelsdorf DJ, Saltiel AR. FGF21 promotes thermogenic gene expression as an autocrine factor in adipocytes. Cell Rep. 2021; 35(13): 109331. PMID 34192547
  36. Ahn SH, Qin S, Zhang JZ, McCammon JA, Zhang J, and Zhou HX. Characterizing protein kinase A (PKA) subunits as macromolecular regulators of PKA RIα liquid-liquid phase separation. J Chem Phys. 2021; 154(22): 221101. PMID 34241191
  37. Bohrer CH, Yang X, Thakur S, Weng X, Tenner B, McQuillen R, Ross B, Wooten M, Chen X, Zhang J, Roberts E, Lakadamyali M, Xiao J. A pairwise Distance Distribution Correction (DDC) algorithm to eliminate blinking-caused artifacts in super-resolution microscopy. Nat Methods. 2021; 18(6): 669-677. PMID 34059826.
  38. Tenner B*, Zhang JZ*, Kwon Y, Pessino V, Feng S, Huang B, Mehta S†, Zhang J†. FluoSTEPs: Fluorescent biosensors for monitoring compartmentalized signaling within endogenous microdomains. *Equal contribution. †Co-corresponding authors. Sci Adv. 2021; 7(21): eabe4091. PMID 34020947.
  39. Mehta S and Zhang J. Biochemical activity architectures visualized – Using genetically encoded fluorescent biosensors to map the spatial boundaries of signaling compartments. Acc Chem Res. 2021; 54(10): 2409-2420. PMID 33949851
  40. Lei Y*, Zhang J*, Schiavon CR, He M, Chen L, Shen H, Zhang Y, Yin Q, Cho Y, Andrade L, Shadel GS, Hepokoski M, Lei T, Wang H, Zhang J, Yuan JXJ, Malhotra A, Manor U†, Wang S†, Yuan Z†, Shyy JY†. SARS-CoV-2 spike protein impairs endothelial function via downregulation of ACE 2. *Equal contribution. †Co-corresponding authors. Circ Res. 2021; 128(9): 1323-1326. PMID 33784827
  41. Roebroek T, Vandenberg W, Sipieter F, Hugelier S, Stove C, Zhang J, Dedecker P. Simultaneous readout of multiple FRET pairs using photochromism. Nat Commun. 2021; 12(1): 2005. PMID 33790271
  42. Lee YU, Zhao J, Ma Q, Khorashad LK, Posner C, Li G, Wisna GBM, Burns Z, Zhang J, Liu Z. Metamaterial assisted illumination nanoscopy via random super-resolution speckles. Nat Commun. 2021; 12(1): 1559. PMID 33692354
  43. Lee YU, Li S, Bopp SE, Zhao J, Nie Z, Posner C, Yang S, Zhang X, Zhang J, Liu Z. Unprecedented fluorophore photostability enabled by low-loss organic hyperbolic materials. Adv Mater. 2021; 33(9): e2006496. PMID 33506542
  44. Lee YU, Posner C, Zhao J, Zhang J, Liu Z. Imaging of cell morphology changes via metamaterial-assisted photobleaching microscopy. Nano Lett. 2021; 21(4): 1716-1721. PMID 33576637
  45. Fong MY, Yan W, Ghassemian M, Wu X, Zhou X, Cao M, Jiang L, Wang J, Liu X, Zhang J, Wang SE. Cancer-secreted miRNAs regulate amino-acid-induced mTORC1 signaling and fibroblast protein synthesis. EMBO Rep. 2021; 22(2): e51239. PMID 33345445
  46. Zhang JF*, Liu B*, Hong I*, Mo A, Roth RH, Tenner B, Lin W, Zhang JZ, Molina RS, Drobizhev M, Hughes TE, Tian L, Huganir RH†, Mehta S†, Zhang J†. An ultrasensitive biosensor for high-resolution kinase activity imaging in awake mice. *Equal contribution. †Co-corresponding authors. Nat Chem Biol. 2021; 17(1): 39-46. PMID 32989297
  47. Zhang JF, Mehta S, Zhang J. Signaling microdomains in the spotlight: visualizing compartmentalized signaling using genetically encoded fluorescent biosensors. Annu Rev Pharmacol Toxicol. 2021; 61: 587-608. PMID 3341579
  48. Zhou X, Zhong Y, Molinar-Inglis O, Kunkel MT, Chen M, Sun T, Zhang J, Shyy JY, Trejo J, Newton AC, Zhang J. Location-specific inhibition of Akt reveals regulation of mTORC1 activity in the nucleus. Nat Commun. 2020; 11(1): 6088. PMID 33257668
  49. Tenner B, Getz M, Ross B, Ohadi D, Bohrer CH, Greenwald E, Mehta S, Xiao J, Rangamani P†, Zhang J†. Spatially compartmentalized phase regulation of a Ca2+-cAMP-PKA oscillatory circuit. †Co-corresponding authors. eLife. 2020; 9: e55013. PMID 33201801
  50. Zhou X, Mehta S, Zhang J. Genetically encodable fluorescent biosensors light up signaling networks. Trends Biochem Sci. 2020; 45(10): 889-905. PMID 32660810
  51. Zhang JZ, Lu TW, Stolerman LM, Tenner B, Yang JR, Zhang JF, Falcke M, Rangamani P, Taylor SS, Mehta S, Zhang J. Phase separation of a regulatory subunit of protein kinase A controls cAMP compartmentation and oncogenic signaling. Cell. 2020; 182(6): 1531-1544. PMID 32846158
  52. Zhang JZ, Nguyen AH, Miyamoto S, Brown JH, McCulloch, AD, Zhang J. Histamine-induced biphasic activation of RhoA allows for persistent RhoA signaling. PLoS Biol. 2020; 18(9): e3000866. PMID 32881857
  53. Lee YU, Zhao J, Mo GCH, Li S, Li G, Ma Q, Yang Q, Lal R, Zhang J, Liu Z. Metamaterial-assisted photobleaching microscopy with nanometer scale axial Resolution. Nano Lett. 2020; 20(8): 6038. PMID 32597659
  54. Keyes J, Ganesan A, Molinar-Inglis O, Hamidzadeh A, Zhang JF, Ling M, Trejo J, Levchenko A, Zhang J. Signaling diversity enabled by Rap1-regulated plasma membrane ERK with distinct temporal dynamics. eLife. 2020; 9: e57410. PMID 32452765
  55. Mo GCH, Posner C, Rodriguez E, Sun T, Zhang J. A rationally enhanced red fluorescent protein expands the utility of FRET biosensors. Nat Commun. 2020; 11(1): 1848. PMID 32296061
  56. Lee HN, Mehta S, Zhang J. Recent advances in the use of genetically encodable optical tools to elicit and monitor signaling events. Curr Opin Cell Biol. 2020; 63: 114-124. PMID 32058267
  57. Wijaya J, Vo BT, Liu J, Xu B, Wu G, Wang Y, Peng J, Zhang J, Janke LJ, Orr BA, Yu J, Roussel MF, Schuetz JD. An ABC transporter drives medulloblastoma pathogenesis by regulating Sonic Hedgehog signaling. Cancer Res. 2020; 80(7): 1524-1537. PMID 31948942
  58. Schmitt DL, Mehta S, Zhang J. Illuminating the kinome: visualizing real-time kinase activity in biological systems using genetically encoded fluorescent protein-based biosensors. Curr Opin Chem Biol. 2020; 54: 63-69. PMID 31911398
  59. Hertel F, Li S, Chen M, Pott L, Mehta S, Zhang J. Fluorescent biosensors for multiplexed imaging of phosphoinositide dynamics. ACS Chem Biol. 2020; 15(1): 33-38. PMID 31855412
  60. Ohadi D, Schmitt DL, Calabrese B, Halpain S, Zhang J, Rangamani P. Computational modeling reveals frequency modulation of calcium-cAMP/PKA pathway in dendritic spines. Biophys J. 2019; 117(10): 1963-1980. PMID: 31668749
  61. Qin Q, Laub S, Shi Y, Ouyang M, Peng Q, Zhang J, Wang Y, Lu. S. Fluocell for ratiometric and high-throughput live-cell image visualization and quantitation. Front Phys. 2019; 7: 00154. PMID 33163483
  62. Lin W, Mehta S, Zhang J. Genetically encoded fluorescent biosensors illuminate kinase signaling in cancer. J Biol Chem. 2019; 294(40): 14814-14822. PMID 31434714
  63. Clister T, Greenwald E, Baillie GS, and Zhang J. AKAP95 organizes a nuclear microdomain to control local cAMP and nuclear PKA activation. Cell Chem Biol. 2019; 26(6): 885-891.e4. PMID 30982750
  64. He M, Huang TS, Li S, Hong HC, Chen Z, Martin M, Zhou X, Huang HY, Su SH, Zhang J, Wang WT, Kang J, Huang HD, Zhang J, Chien S, Shyy JY. Atheroprotective flow upregulates ITPR3 (Inositol 1,4,5-Trisphosphate Receptor 3) in vascular endothelium via KLF4 (Krüppel-Like Factor 4)-mediated histone modifications. Arterioscler Thromb Vasc Biol. 2019; 39(5): 902-914. PMID 30917677
  65. Ross B, Wong SH, Snell NE, Zhang J, Rizzo MA. Triple fluorescence anisotropy reporter imaging in living cells. Bio-protocol. 2019; 9(9): e3226. PMID 31423459
  66. Qian Y, Piatkevich KD, Mc Larney B, Abdelfattah AS, Mehta S, Murdock MH, Gottschalk S, Molina RS, Zhang W, Chen Y, Wu J, Drobizhev M, Hughes TE, Zhang J, Schreiter ER, Shoham S, Razansky D, Boyden ES, Campbell RE. A genetically-encoded near-infrared fluorescent calcium ion indicator. Nat Methods. 2019; 16(2): 171-174. PMID 30664778
  67. Greenwald E, Mehta S, Zhang J. Genetically encoded fluorescent biosensors illuminate the spatiotemporal regulation of signaling networks. Chem Rev. 2018; 118 (24): 11707-11794. PMID 30550275
  68. Hard R, Li N, He W, Ross B, Mo GCH, Peng Q, Stein RL, Komives E, Wang Y, Zhang J, Wang W. Deciphering and engineering chromodomain-methyllysine peptide recognition. Sci Adv. 2018; 4(11): eaau1447. PMID 30417094
  69. Mehta S†*, Zhang Y*, Roth RH*, Zhang JF, Mo A, Tenner B, Huganir RL†, Zhang J†. Single-fluorophore biosensors for sensitive and multiplexed detection of signaling activities. *Equal contribution. †Co-corresponding authors. Nat Cell Biol. 2018; 20(10): 1215-1225. PMID 30250062
  70. Liu SL*, Wang ZG*, Hu Y, Xin Y, Singaram I, Gorai S, Zhou X, Shim Y, Min JH, Gong LW, Hay N, Zhang J, Cho W. Quantitative lipid imaging reveals a new signaling function of phosphatidylinositol-3,4-bisphophate: Isoform- and site-specific activation of Akt. *Equal contribution. Mol Cell. 2018; 71(6): 1092-1104. PMID 30174291
  71. Grimsey NJ, Narala R, Rada CC, Mehta S, Stephens BS, Kufareva I, Lapek J, Gonzalez DJ, Handel TM, Zhang J, Trejo J. A ryrosine switch on NEDD4-2 E3 ligase transmits GPCR inflammatory signaling. Cell Rep. 2018; 24(12): 3312-3323. PMID 30232011
  72. Ross BL, Tenner B, Markwardt ML, Zviman A, Shi G, Kerr JP, Snell NE, McFarland JJ, Mauban JR, Ward CW, Rizzo MA†, Zhang J†. Single-color, ratiometric biosensors for detecting signaling activities. †Co-corresponding authors. eLife. 2018; 7: e35458. PMID 29968564
  73. Chen W*, Yan Z*, Li S*, Huang N, Huang X, Zhang J†, Zhong S†. RNAs as proximity labeling media for identifying nuclear speckle positions relative to the genome. *Equal contribution. †Co-corresponding authors. iScience. 2018; 4: 204-215. PMID 30240742
  74. Oldach LM, Gorshkov K, Mills WT, Zhang J†, Meffert MK†. A biosensor for MAPK-dependent Lin28 signaling. †Co-corresponding authors. Mol Biol Cell. 2018; 29(10): 1157-1167. PMID 29540527
  75. Hertel F, Mo GCH, Dedecker P, Zhang J. Observing the assembly of protein complexes in living eukaryotic cells in super-resolution using refSOFI. Methods Mol Biol. 2018; 1764: 267-277. PMID 29605920
  76. Ni Q, Mehta S, Zhang J. Live-cell imaging of cell signaling using genetically encoded fluorescent reporters. FEBS J. 2018; 285(2): 203-219. PMID 28613457
  77. Chen Y, Booth C, Wang H, Wang RX, Terzi D, Zachariou V, Jiao K, Zhang J, Wang Q. Effective attenuation of adenosine A1R signaling by neurabin requires oligomerization of neurabin. Mol Pharmacol. 2017; 92(6): 630-639. PMID 28954816
  78. Mehta S and Zhang J. Illuminating the cell’s biochemical activity architecture. Biochemistry. 2017; 56(39): 5210-5213. PMID 28718621
  79. Nygren PJ, Mehta S, Schweppe DK, Langeberg LK, Whiting JL, Weisbrod CR, Bruce JE, Zhang J, Veesler D, Scott JD. Intrinsic disorder within AKAP79 fine-tunes anchored phosphatase activity toward substrates and inhibitor drugs. eLife. 2017; 6: e30872. PMID 28967377
  80. Mo GCH, Ross B, Hertel F, Manna P, Yang X, Greenwald E, Booth C, Plummer AM, Tenner B, Chen Z, Wang Y, Kennedy EJ, Cole PA, Fleming KG, Palmer A, Jimenez R, Xiao J, Dedecker P, Zhang J. Genetically encoded biosensors for visualizing live-cell biochemical activity at superresolution. Nat Methods. 2017; 14(4): 427-434. PMID 28288122
  81. Gorshkov K, Mehta S, Ramamurthy S, Ronnett GV, Zhou FQ, Zhang J. AKAP-mediated feedback control of cAMP gradients in developing hippocampal neurons. Nat Chem Biol. 2017; 13(4): 425-431. PMID 28192412
  82. Rodriguez EA, Campbell RE, Lin JY, Lin MZ, Miyawaki A, Palmer AE, Shu X, Zhang J, Tsien RY. The growing and glowing toolbox of fluorescent and photoactive proteins. Trends Biochem Sci. 2017; 42(2): 111-129. PMID 27814948
  83. Newman RH and Zhang J. Integrated strategies to gain a systems-level view of dynamic signaling networks. Methods Enzymol. 2017; 589: 133-170. PMID 28336062
    84. Tenner B, Mehta S, Zhang J. Optical sensors to gain mechanistic insights into signaling assemblies. Curr Opin Struct Biol. 2016; 41: 203-210. PMID 27611602
  84. Zhou X, Li S, Zhang J. Tracking the activity of mTORC1 in living cells using genetically encoded FRET-based biosensor TORCAR. Curr Protoc Chem Biol. 2016; 8(4): 225-233. PMID 27925667
  85. Hung WC*, Yang JR*, Yankaskas CL, Wong BS, Wu PH, Pardo-Pastor C, Serra SA, Chiang MJ, Gu Z, Wirtz D, Valverde MA, Yang JT†, Zhang J†, Konstantopoulos K†. Confinement-sensing and signal optimization via Piezo1/PKA and Myosin II pathways. *Equal contribution. †Co-corresponding authors. Cell Rep. 2016; 15(7):1430-1441. PMID 27160899
  86. Moore BS, Stepanchick AN, Tewson PH, Hartle CM, Zhang J, Quinn AM, Hughes TE, Mirshahi T. Cilia have high cAMP levels that are inhibited by Sonic Hedgehog-regulated calcium dynamics. Proc Natl Acad Sci USA. 2016; 113(46): 13069-13074. PMID 27799542
  87. Conlon P, Gelin-Licht R, Ganesan A, Zhang J, Levchenko A. Single-cell dynamics and variability of MAPK activity in a yeast differentiation pathway. Proc Natl Acad Sci USA. 2016; 113(40): E5896-E5905. PMID 27651485
  88. Ross B, Mehta S, Zhang J. Molecular tools for acute spatiotemporal manipulation of signal transduction. Curr Opin Chem Biol. 2016; 34:135-142. PMID 27639090
  89. Hertel F, Mo GCH, Duwé S, Dedecker P, Zhang J. RefSOFI for mapping nanoscale organization of protein-protein interactions in living cells. Cell Rep. 2016; 14(2): 390-400. PMID 26748717
  90. Depry C, Mehta S, Li R, Zhang J. Visualization of compartmentalized kinase activity dynamics using adaptable bimKARs. Chem Biol. 2015; 22(11): 1470-9. PMID 26548610
  91. Mehta S and Zhang J. Dynamic visualization of calcium-dependent signaling in cellular microdomains. Cell Calcium. 2015. 58(4): 333-41. PMID 25703691
  92. Yaniv Y, Ganesan A, Yang D, Ziman BD, Lyashkov AE, Levchenko A, Zhang J, Lakatta EG. Real-time relationship between PKA biochemical signal network dynamics and increased action potential firing rate in heart pacemaker cells: kinetics of PKA activation in heart pacemaker cells. J Mol Cell Cardiol. 2015; 86: 168-78. PMID 26241846
  93. Hu J, Neiswinger J, Zhang J, Zhu H, Qian J. Systematic prediction of scaffold proteins reveals new design principles in scaffold-mediated signal transduction. PLoS Comput Biol. 2015; 11(9): e1004508. PMID 26393507
  94. Sample V, Ramamurthy S, Gorshkov K, Ronnett GV, Zhang J. Polarized activities of AMPK and BRSK in primary hippocampal neurons. Mol Biol Cell. 2015; 26(10): 1935-1946. PMID 25788287
  95. Karlsson I, Zhou X, Thomas R, Smith AT, Bonner MY, Bakshi P, Banga AK, Bowen JP, Qabaja G, Ford SL, Ballard MD, Petersen KS, Li X, Chen G, Ogretmen B, Zhang J, Watkins EB, Arnold RS, Arbiser JL. Solenopsin A and analogs exhibit ceramide-like biological activity. Vasc Cell. 2015; 7: 5. PMID 26015865
  96. Miyamoto T†, Rho E, Sample V, Akano H, Magari M, Ueno T, Gorshkov K, Chen M, Tokumitsu H, Zhang J†, Inoue T†. Compartmentalized AMPK signaling illuminated by genetically encoded molecular sensors and actuators. †Co-corresponding authors. Cell Rep. 2015; 11(4): 657-670. PMID 25892241
  97. Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GCH, Zhang J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE. Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange. Nat Methods. 2015; 12(3): 195-198. PMID 25622108
  98. Zhou X, Clister TL, Lowry PR, Seldin MM, Wong GW, Zhang J. Dynamic visualization of mTORC1 activity in living cells. Cell Rep. 2015; 10(10): 1767-1777. PMID 25772363
  99. Clister T, Mehta S, and Zhang J. Single-cell analysis of G-protein signal transduction. J Biol Chem. 2015; 290(11): 6681-6688. PMID 25605723
  100. Dou H, Wang C, Wu X, Yao L, Zhang X, Teng S, Xu H, Liu B, Wu Q, Zhang Q, Hu M, Wang Y, Wang L, Wu Y, Shang S, Kang X, Zheng L, Zhang J, Raoux M, Lang J, Li Q, Su J, Yu X, Chen L, Zhou Z. Calcium influx activates adenylyl cyclase 8 for sustained insulin secretion in rat pancreatic beta cells. Diabetologia. 2015; 58(2): 324-233. PMID 25381556
  101. Weng HJ, Patel KN, Jeske NA, Bierbower SM, Zou W, Tiwari V, Zheng Q, Tang Z, Mo GCH, Wang Y, Geng Y, Zhang J, Guan Y, Akopian AN, Dong X. Tmem100 is a regulator of TRPA1-TRPV1 complex and contributes to persistent pain. Neuron. 2015; 85(4): 833-846. PMID 25640077
  102. Gorshkov K and Zhang J. Visualization of cyclic nucleotide dynamics in neurons. Front Cell Neurosci. 2014; 8: 395. PMID 25538560
  103. Newman RH, Zhang J, Zhu H. Toward a systems-level view of dynamic phosphorylation networks. Front Genet. 2014; 5: 263. PMID 25177341
  104. Mehta S, Aye-Han NN, Ganesan A, Oldach L, Gorshkov K, Zhang J. Calmodulin-controlled spatial decoding of oscillatory Ca2+ signals by calcineurin. eLife. 2014; 3: e03865. PMID 25056880
  105. Fu Q, Kim S, Soto D, De Arcangelis V, Dipilato L, Liu S, Xu B, Zhang J, Shi Q, Xiang YK. A long lasting β1 adrenergic receptor stimulation of cAMP/protein kinase A (PKA) signal in cardiac myocytes. J Biol Chem. 2014; 289(21): 14771-14781. PMID 24713698
  106. Haj Slimane Z, Bedioune I, Lechêne P, Varin A, Lefebvre F, Mateo P, Domergue-Dupont V, Dewenter M, Richter W, Conti M, El-Armouche A, Zhang J, Fischmeister R, Vandecasteele G. Control of cytoplasmic and nuclear protein kinase A by phosphodiesterases and phosphatases in cardiac myocytes. Cardiovasc Res. 2014; 102(1): 97-106. PMID 24550350
  107. Wang Y, Ho TG, Bertinetti D, Neddermann M, Franz E, Mo GCH, Schendowich LP, Sukhu A, Spelts RC, Zhang J, Herberg FW, Kennedy EJ. Isoform-selective disruption of AKAP-localized PKA using hydrocarbon stapled peptides. ACS Chem Biol. 2014; 9(3): 635-642. PMID 24422448
  108. Sample V, Mehta S, Zhang J. Genetically encoded molecular probes to visualize and perturb signaling dynamics in living biological systems. J Cell Sci. 2014; 127(Pt 6): 1151-1160. PMID 24634506
  109. Cazabat L, Ragazzon B, Varin A, Potier-Cartereau M, Vandier C, Vezzosi D, Risk-Rabin M, Guellich A, Schittl J, Lechêne P, Richter W, Nikolaev VO, Zhang J, Bertherat J, Vandecasteele G. Inactivation of the Carney complex gene 1 (PRKAR1A) alters spatiotemporal regulation of cAMP and cAMP-dependent protein kinase: a study using genetically-encoded FRET-based reporters. Human Mol Genet. 2014; 23(5): 1163-74. PMID 24122441
  110. Hertel F and Zhang J. Monitoring of post-translational modification dynamics with genetically encoded fluorescent reporters. Biopolymers. 2014; 101(2): 180-187. PMID 23576192
  111. Oldach L and Zhang J. Genetically encoded fluorescent biosensors for live-cell visualization of protein phosphorylation. Chem Biol. 2014; 21(2): 186-197. PMID 24485761
  112. Hu J*, Rho HS*, Newman RH*, Hwang W, Neiswinger J, Zhu H†, Zhang J†, Qian J†. Global analysis of phosphorylation networks in humans. *Equal contribution. †Co-corresponding authors. Biochim Biophys Acta. 2014; 1844(1 Pt B): 224-231. PMID 23524292
  113. Hu J, Rho HS, Newman RH, Zhang J, Zhu H, Qian J. PhosphoNetworks: a database for human phosphorylation networks. Bioinformatics. 2014; 30(1): 141-142. PMID 24227675
  114. Robinson KH, Yang JR, Zhang J. FRET and BRET-based biosensors in live cell compound screens. Methods Mol Biol. 2014; 1071: 217-225. PMID 24052392
  115. Aye-Han NH and Zhang J. Multi-parameter live cell imaging approach to monitor cyclic AMP and protein kinase A dynamics in parallel. Methods Mol Biol. 2014; 1071: 207-215. PMID 24052391
  116. Mehta S and Zhang J. Using a genetically encoded FRET-based reporter to visualize calcineurin phosphatase activity in living cells. Methods Mol Biol. 2014; 1071: 139-149. PMID 24052386
  117. Newman RH and Zhang J. The design and application of genetically encodable biosensors based on fluorescent proteins. Methods Mol Biol. 2014; 1071: 1-16. PMID 24052376
  118. Burdyga A, Conant A, Haynes L, Zhang J, Jalink K, Sutton R, Neoptolemos J, Costello E, Tepikin A. cAMP inhibits migration, ruffling and paxillin accumulation in focal adhesions of pancreatic ductal adenocarcinoma cells: Effects of PKA and EPAC. Biochim Biophys Acta. 2013; 1833(12): 2664-2672. PMID 23797058
  119. Mehta S and Zhang J. Using a kinase-inducible bimolecular switch to control enzyme activity in living cells. Curr Protoc Chem Biol. 2013; 5(3):227-237. PMID 24391085
  120. Woodard CL, Goodwin CR, Wan J, Xia S, Hu J, Newman R, Zhang J, Hayward, SD, Qian J, Laterra J, Zhu H. Profiling the dynamics of a human phosphorylome reveals new components in HGF/c-Met signaling. PLoS One. 2013; 8(9): e72671. PMID 24023761
  121. Nunes AR, Holmes APS, Sample V, Kumar P, Cann MJ, Monteiro EC, Zhang J, Gauda EB. Bicarbonate-sensitive soluble and transmembrane adenylyl cyclases in peripheral chemoreceptors. Respir Physiol Neurobiol. 2013; 188(2): 83-93. PMID 23727159
  122. Huang J, Yang JR, Zhang J†, Yang J†. Total synthesis and structure-activity relationship study of the potent cAMP signaling agonist (−)-Alotaketal A. †Co-corresponding authors. Org Biomol Chem. 2013; 11(19): 3212-3222. PMID 23584129
  123. Depry C, Mehta S, Zhang J. Multiplexed visualization of dynamic signaling networks using genetically encoded fluorescent protein-based biosensors. Pflugers Arch. 2013; 465(3): 373-381. PMID 23138230
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  125. Newman RE*, Hu J*, Rho H*, Xie Z*, Woodard C, Neiswinger J, Cooper C, Shirley M, Clark HM, Hu S, Hwang W, Jeong JS, Wu G, Lin J, Gao X, Ni Q, Goef R, Xia S, Ji H, Dalby KN, Birnbaum MJ, Cole PA, Knapp S, Ryazanov A, Zack DJ, Blackshaw S, Pawson T, Gingras A, Desiderio S, Pandey A, Turk BE, Zhang J†, Zhu H†, Qian J†. Construction of human activity-based phosphorylation networks. *Equal contribution. †Co-corresponding authors. Mol Syst Biol. 2013; 9: 655. PMID 23549483
  126. Dedecker P, Duwé S, Neely RK, Zhang J. Localizer: fast, accurate, open-source, and modular software package for superresolution microscopy. J Biomed Opt. 2012; 17(12): 126008. PMID 23208219
  127. Ghigo A, Perino A, Mehel H, Zahradníková A Jr, Morello F, Leroy J, Nikolaev VO, Damilano F, Cimino J, De Luca E, Richter W, Westenbroek R, Catterall WA, Zhang J, Yan C, Conti M, Gomez AM, Vandecasteele G, Hirsch E, Fischmeister R. Phosphoinositide 3-kinase γ protects against catecholamine-induced ventricular arrhythmia through protein kinase A-mediated regulation of distinct phosphodiesterases. Circulation. 2012; 126(17): 2073-2083. PMID 23008439
  128. Dancy BM, Crump NT, Peterson DJ, Mukherjee C, Bowers EM, Ahn YH, Yoshida M, Zhang J, Mahadevan LC, Meyers DJ, Boeke JD, Cole PA. Live cell studies of p300/CBP histone acetyltransferase activity and inhibition. ChemBioChem. 2012; 13(14): 2113-21. PMID 22961914
  129. Ferrando IM, Chaerkady R, Zhong J, Molina H, Jacob HKC, Herbst-Robinson K, Dancy BM, Katju V, Bose R, Zhang J, Pandey A, Cole PA. Identification of targets of c-Src tyrosine kinase by chemical complementation and phosphoproteomics. Mol Cell Proteomics. 2012; 11(8): 355-69. PMID 22499769
  130. Ganesan A and Zhang J. How cells process information: Quantification of spatiotemporal signaling information. Protein Sci. 2012; 21(7): 918-928. PMID 22573643
  131. Dedecker P, Mo GCH, Dertinger T, Zhang J. Widely accessible method for superresolution fluorescence imaging of living systems. Proc Natl Acad Sci USA. 2012; 109(27): 10909-10914. PMID 22711840
  132. Huang J, Yang JR, Zhang J†, Yang J†. Total synthesis of the potent cAMP signaling agonist (−)-Alotaketal A. †Co-corresponding authors. J Am Chem Soc. 2012; 134(21): 8806-8809. PMID 22563931
  133. Aye-Han NN, Allen MD, Ni Q, Zhang J. Parallel tracking of cAMP and PKA signaling dynamics in living cells with FRET-based fluorescent biosensors. Mol Biosyst. 2012; 8(5): 1435-1440. PMID 22362165
  134. Sample V*, DiPilato LM*, Yang JH*, Ni Q1, Saucerman JJ†, Zhang J†. Regulation of nuclear PKA revealed by spatiotemporal manipulation of cAMP. *Equal contribution. †Co-corresponding authors. Nat Chem Biol. 2012; 8(4): 375-382. PMID 22366721
  135. del Puerto A, Díaz-Hernández JI, Tapia M, Gómez-Villafuertes R, Benitez MJ, Zhang J, Miras-Portugal MT, Wandosell F, Díaz-Hernandez M, Garrido JJ. Adenylate cyclase 5 coordinates the action of ADP, P2Y1, P2Y13, and ATP-gated P2X7 receptors on axonal elongation. J Cell Sci. 2012; 125(Pt 1): 176-188. PMID 22250198
  136. Nunes AR, Sample V, Xiang YK, Monteiro EC, Gauda E, Zhang J. Effect of oxygen on phosphodiesterases (PDE) 3 and 4 isoforms and PKA activity in the superior cervical ganglia. Adv Exp Med Biol. 2012; 758: 287-294. PMID 23080174
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  138. Herbst KJ, Allen MD, Zhang J. Spatiotemporally regulated protein kinase A activity is a critical regulator of growth factor-stimulated extracellular signal-regulated kinase signaling in PC12 cells. Mol Cell Biol. 2011; 31(19): 4063-4075. PMID 21807900
  139. Chakir K*, Depry C*, Dimaano VL, Zhu WZ, Vanderheyden M, Bartunek J, Abraham TP, Tomaselli GF, Liu SB, Xiang YK, Zhang M, Takimoto E, Dulin N, Xiao RP, Zhang J, Kass DA. *Equal contribution. Gαs-biased β2-adrenergic signaling from restoring synchronous contraction in the failing heart. Sci Transl Med. 2011; 3(100): 100ra88. PMID 21918105
  140. Gao X, Lowry PR, Zhou X, Depry C, Wei Z, Wong GW, Zhang J. PI3K/Akt signaling requires spatial compartmentalization in plasma membrane microdomains. Proc Natl Acad Sci USA. 2011; 108(35): 14509-14514. PMID 21873248
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  142. Mehta S and Zhang J. Reporting from the field: Genetically encoded fluorescent reporters uncover signaling dynamics in living biological systems. Annu Rev Biochem. 2011; 80: 375-401. PMID 21495849
  143. Newman RH, Fosbrink MD, Zhang J. Genetically encoded fluorescent biosensors for tracking signaling dynamics in living cells. Chem Rev. 2011; 111(5): 3614-3666. PMID 21456512
  144. Herbst KJ, Allen MD, Zhang J. Luminescent kinase activity biosensors based on a versatile molecular switch. J Am Chem Soc. 2011; 133(15): 5676-5679. PMID 21438554
  145. Wan M, Li J, Herbst K, Zhang J, Yu B, Wu X, Qiu T, Lei W, Lindvall C, Williams BO, Ma H, Zhang F, Cao X. LRP6 mediates cAMP generation by G protein-coupled receptors through regulating the membrane targeting of Gα(s). Sci Signal. 2011; 4(164): ra15. PMID 21406690
  146. Herbst KJ, Coltharp C, Amzel LM, Zhang J. Direct activation of Epac by sulfonylurea is isoform selective. Chem Biol. 2011; 18(2): 243-251. PMID 21338921
  147. Ni Q*, Ganesan A*, Aye-Han NN*, Gao X, Allen MD, Levchenko A†, Zhang J†. Signaling diversity of PKA achieved via a Ca2+-cAMP-PKA oscillatory circuit. *Equal contribution. †Co-corresponding authors. Nat Chem Biol. 2011; 7(1): 34-40. PMID 21102470Depry C, Allen MD, Zhang J. Visualization of PKA activity in plasma membrane microdomains. Mol Biosyst. 2011; 7(1): 52-58. PMID 20838685
  148. Liu S, Zhang J, Y. Xiang. FRET-based direct detection of dynamic protein kinase A activity on the sarcoplasmic reticulum in cardiomyocytes. Biochem Biophys Res Commun. 2011; 404(2): 581-586. PMID 21130738
  149. Gildea JJ, Shah I, Weiss R, Casscells ND, McGrath HE, Zhang J, Jones JE, Felder RA. HK-2 human renal proximal tubule cells as a model for G protein-coupled receptor kinse type 4-mediated dopamine 1 receptor uncoupling. Hypertension. 2010; 56(3): 505-11. PMID 20660820
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  151. Yamashiro T, Kuge H, Zhang J, Honke K. Calcineurin mediates the angiotensin II-induced aldosterone synthesis in the adrenal glands by upregulation of transcription of the CYP11B2 gene. J Biochem. 2010; 148(1): 115-123. PMID 20413672
  152. Fosbrink M, Aye-Han NN, Cheong R, Levchenko A, Zhang J. Visualization of JNK activity dynamics with a genetically encoded fluorescent biosensor. Proc Natl Acad Sci USA. 2010; 107(12): 5459-5464. PMID 20212108
  153. DiPilato LM and Zhang J. Fluorescent protein-based biosensors: resolving spatiotemporal dynamics of signaling. Curr Opin Chem Biol. 2010; 14(1): 37-42. PMID 19910237
  154. Gao X and Zhang J. FRET-based activity biosensors to probe compartmentalized signaling. ChemBioChem. 2010; 11(2): 147-151. PMID 20014085
  155. Gildea JJ, Israel JA, Johnson AK, Zhang J, Jose PA, Felder RA. Caveolin-1 and dopamine-mediated internalization of NaKATPase in human renal proximal tubule cells. Hypertension. 2009; 54(5): 1070-1076. PMID 19752292
  156. Aye-Han NN, Ni Q, Zhang J. Fluorescence biosensors for real-time tracking of post-translational modification dynamics. Curr Opin Chem Biol. 2009; 13(4): 392-397. PMID 19682946
  157. Sample V, Newman RH, Zhang J. The structure and function of fluorescent proteins. Chem Soc Rev. 2009; 38(10): 2852-2864. PMID 19771332
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  159. DiPilato LM and Zhang J. The role of membrane microdomains in shaping β2-adrenergic receptor-mediated cAMP dynamics. Mol Biosyst. 2009; 5(8): 832-837. PMID 19603118
  160. Herbst KJ, Allen MD, Zhang J. The cAMP-dependent protein kinase inhibitor H-89 attenuates the bioluminescent signal produced by Renilla luciferase. PLoS One. 2009; 4(5): e5642. PMID 19461967
  161. Soto D, De Arcangelis V, Zhang J, Xiang Y. Dynamic protein kinase A activities induced by β adrenoceptors dictate signaling propagation for substrate phosphorylation and myocyte contraction. Circ Res. 2009; 104(6): 770-9. PMID 19213958
  162. Seong J, Lu S, Ouyang M, Huang H, Zhang J, Frame MC, Wang Y. Visualization of Src activity at different compartments of the plasma membrane by FRET imaging. Chem Biol. 2009; 16(1): 48-57. PMID 19171305
  163. Rahdar M, Inoue T, Meyer T, Zhang J, Vazquez F, Devreotes PN. A phosphorylation-dependent intramolecular interaction regulates the membrane association and activity of the tumor suppressor PTEN. Proc Natl Acad Sci USA. 2009; 106(2): 480-485. PMID 19114656
    164. Ren YR, Pan F, Parvez S, Fleig A, Chong CR, Xu J, Dang Y, Zhang J, Jiang H, Penner R, Liu JO. Clofazimine inhibits human Kv1.3 potassium channel by perturbing calcium oscillation in T lymphocytes. PLoS One. 2008; 3(12): e4009. PMID 19104661
  164. Lim CJ, Kain KH, Tkachenko E, Goldfinger LE, Gutierrez E, Allen MD, Groisman A, Zhang J, Ginsberg MH. Integrin-mediated protein kinase A activation at the leading edge of migrating cells. Mol Biol Cell. 2008; 19(11): 4930-4941. PMID 18784251
  165. Vincent P, Gervasi N, Zhang J. Real-time monitoring of cyclic nucleotide signaling in neurons using genetically encoded FRET probes. Brain Cell Biol. 2008; 36: 3-17. PMID 18941898
  166. Gao X and Zhang J. Spatiotemporal analysis of differential Akt regulation in plasma membrane microdomains. Mol Biol Cell. 2008; 19(10): 4366-4373. PMID 18701703
  167. Szewczuk LM, Tarrant MK, Sample V, Drury WJ 3rd, Zhang J, Cole PA. Analysis of serotonin N-acetyltransferase regulation in vitro and in live cells using protein semisynthesis. Biochemistry. 2008; 47(39): 10407-10419. PMID 18771288
  168. Lu S, Ouyang M, Seong J, Zhang J, Chien S, Wang Y. The spatiotemporal pattern of Src activation at lipid rafts revealed by diffusion-corrected FRET imaging. PLoS Comput Biol. 2008; 4(7): e1000127. PMID 18711637
  169. Newman RH and Zhang J. Visualization of phosphatase activity in living cells with a FRET-based calcineurin activity sensor. Mol Biosyst. 2008; 4(6): 496-501. PMID 18493642
  170. Violin JD*, DiPilato LM*, Yildrim N*, Elston TC†, Zhang J†, Lefkowitz RJ†. β2-adrenergic receptor signaling and desensitization elucidated by quantitative modeling of real-time cAMP dynamics. *Equal contribution. †Co-corresponding authors. J Biol Chem. 2008; 283(5): 2949-2961. PMID 18045878
  171. Allen MD and Zhang J. A tunable FRET circuit for engineering fluorescent biosensors. Angew Chem Int Ed Engl. 2008; 47(3): 500-502. PMID 18058965
  172. Ananthanarayanan B, Ni Q, Zhang J. Molecular sensors based on fluorescence resonance energy transfer to visualize cellular dynamics. Methods Cell Biol. 2008; 89: 37-57. PMID 19118671
  173. Ananthanarayanan B, Fosbrink M, Rahdar M, Zhang J. Live-cell molecular analysis of Akt activation reveals roles for activation loop phosphorylation. J Biol Chem. 2007; 282(50): 36634-36641. PMID 17928291
  174. Zhang J and Allen MD. FRET-based biosensors for protein kinases: illuminating the kinome. Mol Biosyst. 2007; 3(11): 759-765. PMID 17940658
  175. Sachs BD, Baillie GS, McCall JR, Passino MA, Schachtrup C, Wallace DA, Dunlop AJ, Mackenzie KF, Klussmann E, Lynch MJ, Sikorski SL, Nuriel T, Tsigelny I, Zhang J, Houslay MD, Chao MV, Akassoglou K. p75 neurotrophin receptor regulates tissue fibrosis through inhibition of plasminogen activation via a PDE4/cAMP/PKA pathway. J Cell Biol. 2007; 177(6): 1119-1132. PMID 17576803
  176. Maresh J, Zhang J, Tzeng YL, Goodman NA, Lynn DG. Rational design of inhibitors of VirA-VirG two-component signal transduction. Bioorg Med Chem Lett. 2007; 17: 3281-3286. PMID 17470391
  177. Gervasi N, Hepp R, Tricoire L, Zhang J, Lambolez B, Paupardin-Tritsch D, Vincent P. Dynamics of protein kinase A signaling at the membrane, in the cytosol, and in the nucleus of neurons in mouse brain slices. J Neurosci. 2007; 27(11): 2744-2750. PMID 17360896
  178. Dunn T, Wang CT, Colicos MA, Zaccolo M, DiPilato LM, Zhang J, Tsien RY, Feller MB. Imaging of cAMP levels and PKA activity reveals that retinal waves drive oscillations in second messenger cascades. J Neurosci. 2006; 26(49):12807-12815. PMID 17151284
  179. Ni Q, Titov DV, Zhang J. Analyzing protein kinase dynamics in living cells with FRET reporters. Methods. 2006; 40: 279-286. PMID 16908183
  180. Allen MD and Zhang J. Subcellular dynamics of protein kinase A activity visualized by FRET-based reporters. Biochem Biophys Res Commun. 2006; 348(2): 716-721. PMID 16895723
  181. Saucerman JJ, Zhang J, Martin JC, Peng LX, Stenbit AE, Tsien RY, McCulloch AD. Systems analysis of PKA-mediated phosphorylation gradients in cardiac myocytes. Proc Natl Acad Sci USA. 2006; 103(34): 12923-12928. PMID 16905651
  182. Allen MD, DiPilato LM, Rahdar M, Ren YR, Chong C, Liu JO, Zhang J. Reading dynamic kinase activity in living cells for high-throughput screening. ACS Chem Biol. 2006; 1(6): 371-376. PMID 17163774
  183. Maresh J*, Zhang J*, Lynn DG. The Innate Immunity of maize and the dynamic chemical strategies tegulating two-component signal transduction in Agrobacterium tumefaciens. *Equal contribution. ACS Chem Biol. 2006; 1(3): 165-175. PMID 17163664
  184. Qiao Y, Molina H, Pandey A, Zhang J, Cole PA. Chemical rescue of a mutant enzyme in living cells. Science. 2006; 311(5765): 1293-1297. PMID 16513984
  185. Ananthanarayanan B, Ni Q, Zhang J. Signal propagation from membrane messengers to nuclear effectors revealed by reporters of phosphoinositide dynamics and Akt activity. Proc Natl Acad Sci USA. 2005; 102(42): 15081-15086. PMID 16214892
  186. Zhang J*, Hupfeld CJ*, Taylor SS, Olefsky JM, Tsien RY. Insulin disrupts beta-adrenergic receptor signaling to protein kinase A in adipocytes. *Equal contribution. Nature. 2005; 437(7058): 569-573. PMID 16177793
  187. Kunkel MT, Ni Q, Tsien RY, Zhang J, Newton AC. Spatio-temporal dynamics of protein kinase B/Akt signaling revealed by a genetically encoded fluorescent reporter. J Biol Chem. 2005; 280(7): 5581-5587. PMID 15583002
  188. DiPilato LM, Cheng X, Zhang J. Fluorescent indicators of cAMP and Epac activation reveal differential dynamics of cAMP signaling within discrete subcellular compartments. Proc Natl Acad Sci USA. 2004; 101(47): 16513-16518. PMID 15545605
  189. Violin JD, Zhang J, Tsien RY, Newton AC. A genetically encoded fluorescent reporter reveals oscillatory phosphorylation by protein kinase C. J Cell Biol. 2003; 161(5): 899-909. PMID 12782683
  190. Zhang J, Campbell RE, Ting AY, Tsien RY. Creating new fluorescent probes for cell biology. Nat Rev Mol Cell Biol. 2002; 3(12): 906-918. PMID 12461557
  191. Zhang J, Ma Y, Taylor SS, Tsien RY. Genetically encoded reporters of protein kinase A activity reveal impact of substrate tethering. Proc Natl Acad Sci USA. 2001; 98(26): 14997-15002. PMID 11752448
  192. Zhang J, Boone L, Kocz R, Zhang C, Binns AN, Lynn DG. At the maize/Agrobacterium interface: natural factors limiting host transformation. Chem Biol. 2000; 7(8): 611-621. PMID 11048952
  193. Campbell AM, Tok JB, Zhang J, Wang Y, Stein M, Lynn DG, Binns AN. Xenognosin sensing in virulence: Is there a phenol receptor in Agrobacterium tumefaciens? Chem Biol. 2000; 7(1): 65-76. PMID 10662683
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